Ricardo Rocha is an injury doubt for the hosts after the Portuguese defender missed last Saturday's 3-2 defeat by Brentford with a hamstring problem.
Sam Sodje stepped into the back four and will keep his place if Rocha is ruled out again.
Caretaker boss Guy Whittingham has no other injury concerns and could stick with the side narrowly beaten by the promotion-chasing Bees.
However, with Pompey's relegation having been confirmed in midweek, Whittingham may opt to give some of the club's youngsters such as Jack Maloney and Nick Awford a chance.
Midfielder Liam Walker pressed his claims for a return to the starting line-up with a goal in a midweek friendly against Icelandic side IBV.
The visitors will be without suspended defender Harry Maguire after the centre-back was sent off for two yellow card offences in the midweek draw with Brentford and sits out a one-game ban, so Danny Higginbotham is expected to start alongside Neill Collins at the heart of defence.
Manager Chris Morgan, who has no other new injury or suspension worries, must decide whether to make further changes as his side look to close the five-point gap between themselves and the second automatic promotion place.
Midfielder Barry Robson, who stepped off the bench to score a penalty in midweek, and striker Jonathan Forte are both pushing for recalls, but Chris Porter is expected to continue up front alongside Dave Kitson.
The Blades cannot afford to lose ground on the two teams above them, Brentford and Bournemouth, with only three games remaining.