Joe Martin will miss Gillingham's home clash against the Dons having been ruled out of the remaining two games of the season with a fractured toe.
The left-back was forced off at half-time in last weekend's 1-0 defeat at Cheltenham and will now play no part against the Dons as the Gills go in search of the point which will crown them League Two champions.
Centre-back Leon Legge is only rated as 50/50 due to a hip injury and will hope to be involved at Priestfield, while Myles Weston (ankle) also continues to be assessed.
Already-promoted Gillingham are six points clear of second-placed Port Vale with two matches remaining and know any slip-up by the Valiants at home to play-off hopefuls Northampton will also be enough to seal top spot.
Gary Alexander could return to the AFC Wimbledon squad.
The experienced striker missed last Saturday's 2-2 draw against Exeter after suffering an ankle injury the previous weekend, but he is due back in training ahead of the trip to Kent.
Midfielder Peter Sweeney has resumed running after eight weeks out with a fractured metatarsal but will not be ready in time.
Full-back Jim Fenlon has also stepped up his training following two months on the sidelines with a prolapsed disc in his lower back, but he will not be involved in the Dons' final two matches.
Defender Mat Mitchel-King managed an hour on Saturday despite a back injury and should keep his place, although Will Antwi is on standby.