Rohan Ince and Gavin Williams are manager Gary Johnson's only injury concerns. Ince joined the Glovers on a six-month loan deal from Chelsea in July, but is doubtful for Tuesday's game after being helped from the pitch with a shin injury in a pre-season friendly against Newport.
Ince was joined by Williams on the sidelines for Yeovil's final warm-up match against Exeter, with the Welsh midfielder left out due to a calf injury.
The squad includes a number of new players with Gozie Ugwu, Byron Webster, Marek Stech, Nathan Ralph, Lewis Young, James Hayter and Jamie McAllister all making the move to Huish Park.
Ben Coker will be assessed. The left-back sustained a thigh injury in Saturday's 1-0 friendly victory over Bishop's Stortford and faces a race against time to be fit for the first-round tie.
Manager John Ward told the Daily Gazette: "Ben's injury was unavoidable. He's got a dead leg after going into a challenge that was fair and honest.
"Both players wanted to win the ball but Ben got caught on the thigh. They are awkward ones; they can either clear up quickly or take a couple of weeks."
Otherwise, Ward is not expected to have any other injury concerns for the encounter as the U's look to make it through to the second round of the competition for the third successive season.