Cheltenham have no fresh injury worries as they look to close the one-point gap on the automatic promotion places.
The Robins ran out 2-1 winners against struggling AFC Wimbledon on Saturday and manager Mark Yates is unlikely to make any significant changes to his squad.
Midfielder Sam Deering has impressed Yates since returning to the side and he is likely to get the nod from the start again as they search for back-to-back victories for the first time since November.
"We're starting to show glimpses of our true form, the players understand what we're after," Yates told the club's official website.
"We want them to pass the ball, hurt teams, score goals and keep clean sheets. Saturday was a really good step in the right direction and hopefully we can build on that result."
Chesterfield claimed a 4-1 winner when the sides last met back in November.
Chesterfield boss Paul Cook hopes to have Jack Lester available.
The veteran striker missed Saturday's trip to Dagenham with a virus and faces a late check.
However, Cook may opt to name an unchanged side having secured a 1-0 win at Victoria Road.
Skipper Sam Togwell is expected to continue in midfield having made a successful return from a hamstring problem, while forward Armand Gnanduillet also impressed on his first start for the club.
Cook has no other injury or suspension worries with only Craig Clay set for a spell on the sidelines as he continues to receive treatment on a hernia problem.
The Spireites have won just three of their 10 league matches since the turn of the year and have fallen 10 points adrift of the play-off places.