Bristol Rovers manager Mark McGhee is expected to ring the changes.
Rovers have yet to win a game this season and McGhee is expected to take the opportunity to try new ideas.
Loanee Cian Bolger is expected to be handed a debut in the heart of the defence after his deadline day move from Leicester.
Summer-signing Fabian Broghammer is expected to be handed his first start in attack after three substitute appearances so far this season.
Oliver Norburn, Joe Anyinsah, Seanus Clucas and Matthew Lund are also pushing for a place in the starting line-up.
Whoever is selected to start, McGhee will expect a reaction from Saturday's 3-0 defeat at home to Morecambe, where he kept the players back at the ground after the full-time whistle to watch a DVD of the game.
Yeovil are unlikely to have Gavin Williams available.
The midfielder has been struggling all season with a calf problem and is doubtful to return against Rovers.
Manager Gary Johnson could make a couple of changes for the game.
On-loan midfielder Rohan Ince could make his full debut after being named on the bench in the last two games.
The Chelsea youngster has been unable to break into the starting XI after recovering from a shin problem but could be handed a chance to impress in the trophy.
Fellow loanee Gozie Ugwu has scored three goals in the club's last two league games after coming off the bench and will hope to show what he can do from the start.