Leyton Orient will be without strikers Kevin Lisbie and Charlie MacDonald for the first leg of their southern area final encounter.
Top scorer Lisbie is still out with a hamstring injury and recent signing MacDonald is cup-tied having already played in the competition for MK Dons.
Millwall loanee Shaun Batt, who made his debut in Saturday's 1-0 win at Stevenage, is eligible to play and will partner David Mooney in attack.
Martin Rowlands and Lee Cook were rested at the weekend, although the latter came on as a substitute, and both should start against the Shrimpers.
Defender Nathan Clarke could also be involved after making his long-awaited return from an ankle injury as a late sub.
Southend are without Gavin Tomlin, Marc Laird and Michael Spillane for the midweek tie.
Leading scorer Tomlin missed the weekend victory over Oxford after sustaining a thigh problem in training on Friday and is scheduled for a scan on Tuesday to assess the damage.
Laird misses the game against his former club as his late substitute appearance for Orient in round two means he is cup-tied.
And fellow midfielder Spillane is also ineligible after scoring for Dagenham in their win against Stevenage earlier in the competition.
Ryan Leonard could come into the side after he came off the bench to score the winner against Oxford - his first goal since August 2011 - while on-loan midfielder Ben Reeves could be handed his full debut.