The 28-year-old Manchester United winger has been suffering with an ankle problem and missed Sunday's Community Shield win over Wigan.
A Football Association statement said there had been discussions between England medical staff and their counterparts at Old Trafford and Young had returned to his club.
The main question marks for England surround the fitness of Wayne Rooney and Jack Wilshere, with both looking like possible starters.
Rooney has been struggling with hamstring and shoulder injuries that have ruled him out of most of Manchester United's pre-season programme, but he has been involved in training this week, with Roy Hodgson hinting that the striker could start.
Hodgson has promised Arsene Wenger that he will not play Wilshere for the whole 90 minutes against Scotland. The midfielder has played some part in all but two of Arsenal's pre-season matches as he builds up his fitness for the new season, so looks likely to play for just 45 minutes on Wednesday night.
Southampton striker Rickie Lambert will hope to have impressed Hodgson in training this week and could earn his first cap off the bench.
Liam Bridcutt, who made his one and only Scotland appearance against Serbia in March and is suffering from a groin problem, is the only absentee from Gordon Strachan's original pool of 29 players.
With another 13 midfielders at his disposal, Strachan has not added to his larger than usual squad.
A host of regulars return after missing June's victory in Croatia, including Celtic captain Brown and experienced striker Kenny Miller.
However, skipper Darren Fletcher, defender Gary Caldwell and striker Steven Fletcher remain sidelined for the Scots.