Brentford have no new injury worries for the end-of-season showpiece.
The Bees have also secured an extension to Jake Bidwell's loan stay from Everton, allowing him to play at Wembley.
The 20-year-old played both legs of the semi-final victory against Swindon and will start at left-back.
Back-up goalkeeper Richard Lee remains sidelined after a shoulder operation and defender Scott Barron is recovering from hip surgery, but the rest of Rosler's squad is fit.
Rosler rotated his squad over the two legs so the likes of captain Kevin O'Connor, fellow midfielder Jonathan Douglas, winger Sam Saunders and striker Bradley Wright-Phillips will be in the reckoning for Wembley recalls.
Gary Johnson is hopeful of taking a fully-fit Yeovil squad to the home of English football.
Defensive trio Byron Webster, Jamie McAllister and Dan Burn all returned from injury to feature in the semi-finals against Sheffield United but the latter had an injury scare in midweek at the hands of top scorer Paddy Madden.
The 23-goal striker was reversing out of his parking space after training and failed to realise the Fulham loanee still had his leg outside the passenger door.
The damage was just a tyre mark up an angry team-mate's leg, much to the relief of their manager.
"The only problem we had is that Paddy Madden has just passed his test and he drove over Dan Burn's ankle which is unbelievable," he explained. "He nearly put our centre half out of action with a bad bit of driving."
Nathan Ralph's knee injury means he is the only real worry for Johnson ahead of kick off as the Glovers look to clinch promotion to the second tier of English football for the first time in their history.