Rovers have endured a difficult start to the season, following up a draw to Derby with three successive defeats and they are without seven members of their first-team squad against the Tykes.
Forward Ruben Rochina (shoulder) is months away from a return while former England goalkeeper Paul Robinson (back) and midfielder Dickson Etuhu (knee) are still some way off coming back into the reckoning.
They are, however, making good progress, as is striker DJ Campbell (ankle) and defender Adam Henley (hamstring). Midfielder Lee Williamson (back) could be back by the end of the month while summer signing Matt Killgallon (groin) awaits his first appearance.
Meanwhile, Barnsley's new signing Marcus Pedersen is hoping to make his debut for the club at Blackburn.
The Norway international joined on loan until the end of the season from Dutch side Vitesse Arnhem on Thursday and will be included in David Flitcroft's squad,
subject to international clearance.
Another new signing, former Rochdale and Celtic winger Paddy McCourt, is not expected to feature.
Defender Lewin Nyatanga (hamstring) is hoping to make his first appearance since returning to the club in the summer, but Martin Cranie (knee) remains sidelined.
Midfielder Kelvin Etuhu is pushing for a start after making his first appearance of the season off the bench against Charlton last week following a hamstring injury.