That's all from this minute-by-minute commentary - check back for a match report and reaction. Thanks for joining me.
Full-time at the Maracana: Colombia 2 (James Rodriguez 28, 50) Uruguay 0. Colombia will face Brazil in the second quarter-final in Fortaleza next Friday.
Three added minutes to be played.
Ospina claims Gaston Ramirez's free-kick from long distance.
Third and final change of the night for Colombia - Adrian Ramos ON, James Rodriguez OFF. Standing ovation for the two-goal forward (85).
Ospina has to make another save, this time low to his right, to keep out Cavani's scuffed shot.
Second change for Colombia - Guarin ON, Cuadrado OFF (80).
Ospina saves well from Maxi Pereira after the ball ran kindly for the Uruguay right-back.
YELLOW CARD! Armero is booked as well for his part in all that.
YELLOW CARD! Referee Kuipers has booked injured Uruguay captain Diego Lugano for his protestations from the bench. That was all to do with Ramirez's rather wild challenge on Armero on the touchline. The Colombian needs treatment, but he'll continue (77).
Colombia's possession count is down to 57% now as Uruguay get to see more of the ball. On shots, Tabarez's team lead 12 to nine - but Colombia have four on target to Uruguay's two.
Cavani flicks a header from a corner over Ospina's crossbar.
First substitution for Colombia - Mejia ON, Gutierrez OFF (68).
Third and final change for Uruguay - Abel Hernandez ON, Alvaro Gonzalez OFF (67).
Cristian Rodriguez tries to make it happen for Uruguay with a fierce shot from distance after driving through the midfield - but Ospina dives to his left to make the save.
Not only is James Rodriguez leading the race for the Golden Boot, his Colombia colleague Juan Cuadrado now has the most assists in the tournament, with four.
James Rodriguez goes down clutching his foot after a sneaky challenge from Cavani.
YELLOW CARD! Uruguay's teenage centre-back Jose Maria Gimenez is the first player booked by referee Bjorn Kuipers, for a late slide tackle on Teofilo Gutierrez (55).
Uruguay coach Tabarez is making changes, unsurprisingly. Stuani and Gaston Ramirez ON, Forlan and Alvaro Pereira OFF (53).
It's that man again! Gutierrez starts the move on the right before play is spread to Armero on the other flank. His deep cross is headed back and down by Cuadrado to the edge of the six-yard box where James Rodriguez is there to sweep home his fifth goal of the tournament. He's now the World Cup's leading goalscorer.
GOAL! Colombia 2 (James Rodriguez 50) Uruguay 0
Ospina makes a good take on a corner as Uruguay mount some early second-half pressure.
Remember, the winners of this game will face hosts Brazil in Fortaleza on Friday night in the second of the quarter-finals.
Colombia get the second half underway.
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What did you make of the first half? Colombia have bossed possession (65% to 35%) and have had seven shots (three on target) to Uruguay's four (none on target).
Half-time at the Maracana: Colombia 1 Uruguay 0. James Rodriguez's outstanding 28th-minute volley separates the teams in Rio de Janeiro.
One minute of added-on time to be played.
Armero clears the ball on the edge of Colombia's box after good build-up play from Uruguay involving Forlan.
Ospina is called upon to make a diving save forwards as Gonzalez's shot takes a slight nick on its way towards him.
Referee Kuipers has to have a word with Yepes and Forlan who have had a heated argument in the penalty area.
Great ball in from the left flank by Alvaro Pereira, and it's put behind for a corner.
Cavani goes close with a free-kick attempt from almost 30 yards out, but the ball dips and swerves just over the bar. Uruguay better since falling behind.
Martinez is there on the defensive duties again, heading away a dangerous Uruguay corner.
A stunning goal from James Rodriguez, who takes Aguilar's pass on his chest 22 yards out and volleys a shot left-footed over the fingertips of Muslera, then sees the ball bounce down in off the bar! Stunning - a goal-of-the-tournament contender.
GOAL! Colombia 1 (James Rodriguez 28) Uruguay 0
Aguilar is having to take a break off the pitch briefly after sustaining a slight knock.
Cuadrado and Zuniga continue to be the lively performers for Colombia but they're not doing much to really threaten Uruguay.
Half a chance for Cavani as he races onto a long ball in the box but he can't keep the ball in play.
Colombia have had a whopping 71% possession in the opening 17 minutes of this game. But can they break Uruguay down?
Zuniga is on the floor once again after a rugged Uruguay challenge. He's had an outstanding start to the game.
Ambitious effort from Forlan as Yepes put pressure on him 25 yards out, the ball sailing high and wide.
Muslera has to make a save low down to his left as Zuniga races forward from the halfway line and strikes a shot from distance.
Jackson Martinez heads the ball away as Uruguay mount an attack.
Now Zuniga goes down under a challenge by Cavani. Tasty start to this one.
Some silky step-overs from Cuadrado who is brought down by a combination of Arevalo Rios and Alvaro Pereira.
Dutch referee Bjorn Kuipers has to have an early word with Cristian Rodriguez.
Uruguay are indeed going for three at the back, with the Pereiras - Maxi and Alvaro - bombing down the right and left flanks respectively.
Uruguay kick off the game at the Maracana.
The national anthems are in progress and we'll be underway shortly...
On email, Keith O'Connor is bucking the trend: "I think Uruguay may surprise everyone tonight. They have a point to prove against FIFA - and the best way to do it is by lifting the trophy or at the very least, getting to the final. I reckon 3-2 to Uruguay in extra-time."
Here's some Sky Bet Price Boosts for you. Colombia's Juan Cuadrado to score the first goal is now 12/1; a 2-0 Colombia win in normal time is 9/1; and a 2-1 Uruguay victory within 90 minutes is a tempting 16/1.
Also on email, Chris Green predicts who will be setting up a clash with Brazil next Friday night: "Colombia to win 2-1."
Jordan Green on email is looking forward to this one: "Let's see how Uruguay do without Suarez. They were poor against England - only two shots on target, and Suarez scored the two."
Let's look at the betting. Colombia are 11/10 favourites to win this one within normal time, while you can get 14/5 on Uruguay. The draw at 90 minutes is 23/10.
Colombia and Uruguay's only previous World Cup encounter was in the group stages of the 1962 tournament. The Celeste won 2-1, despite Colombia opening the scoring. It is Colombia's only previous game against a fellow South American team at the World Cup.
Our man Charlie Nicholas has had a look at this game and is going for a 3-1 win to Colombia: "Without Suarez, I don't really see how Uruguay are going to a stop a quick and rugged Colombia team in this game."
It'd be great to hear your feedback on this one. You can get in touch with me throughout the evening by emailing email@example.com or tweet me at @jonboy79.
So Oscar Tabarez selects Diego Forlan to replace the banned Luis Suarez in attack. Nicolas Lodeiro also drops out of the side that began the 1-0 Group D win over Italy, with Maxi Pereira returning at right wing-back.
Uruguay starting line-up (3-4-2-1): Muslera; Gimenez, Godin (c), Caceres; M Pereira, Arevalo Rios, Gonzalez, A Pereira; Forlan, C Rodriguez; Cavani.
A slight surprise there from Colombia coach Jose Pekerman, who selects Jackson Martinez ahead of Victor Ibarbo. Those two goals against Japan last time out have made a big impression. He's made seven changes in total from that game.
Colombia starting line-up (4-2-3-1): Ospina; Zuniga, Zapata, Yepes, Armero; Aguilar, Sanchez; Cuadrado, Rodriguez, Martinez; Gutierrez.
The teams are in...
The two teams now know they are battling for the right to face hosts Brazil in the second quarter-final in Fortaleza next Friday. That's after Luis Felipe Scolari's side thrillingly beat Chile 3-2 on penalties after a 1-1 draw.
Welcome along to our live coverage of the World Cup round-of-16 clash between Colombia and Uruguay at the Maracana in Rio de Janeiro.