Chelsea have submitted two unsuccessful bids for Rooney, who has not featured for United in their pre-season games although he did start for England in Wednesday's friendly victory over Scotland.
Speaking at the Premier League launch in London, Moyes told Sky Sports News: "There have been no more developments and we don't want any more developments because we have told everybody - which I have said before - he is not for sale."
Rooney, who has been suffering with a shoulder injury, could now feature for United in their Premier League opener at Swansea on Saturday - live on Sky Sports - and Moyes will assess the striker's fitness when he returns to the club from international duty.
Moyes, who was speaking at the launch of the new Premier League season, added: "I will need to see how he is when we get back. When I get back I will try and assess him.
"I watched the game last night and I thought for the 65 minutes or so that he played, I thought he did well.
"He ran around, he got himself some fitness, but it certainly was his first outing in any real competitive game."