Manager Chris Coleman has seen captain Ashley Williams pull out of the squad with an ankle injury and called 30-year-old Collins to the Vale of Glamorgan training complex on Wednesday.
Collins has been out of the international picture since allegedly refusing a call-up for a clash with Serbia in September, but Coleman insists the issue is now dead and buried.
Coleman said: "I was disappointed in James. I like James, he's a good man, but my job and how I see it and with the importance of players turning up for any Wales game - I didn't like that.
"I told James that on the phone. I wasn't happy that he wouldn't be considered as a stand-by to be called into the squad. My stance hasn't changed on that and it won't change. He got it wrong in my eyes."
Promising Liverpool youngster Harry Wilson, just 16, has also been added to the group having impressed during U17 games against Hungary, Scotland and Slovenia.
Adam Matthews, Ben Davies, Gareth Bale, Danny Gabbidon, Sam Ricketts, Jack Collison, Joe Allen, Shaun MacDonald and Joe Ledley are also unavailable to Coleman, whose side sit rock bottom of Group A.
Following the Macedonia clash at Cardiff City Stadium, Wales travel away for arguably the toughest fixture of their campaign - facing Belgium in Brussels on Tuesday.