Speculation started on Saturday which suggested the former Spurs player was turning out for the Rs in a behind-closed-doors game against Leyton Orient.
But O'Hara, who has been told he does not feature in new manager Kenny Jackett's plans at Molineux, insists he was not involved with Harry Redknapp's side.
He told the Express and Star: "I have had so many text messages and phone calls about it. The rumour that I was there on trial was not true - I was out with my kids at the time.
"Nothing has happened - I'm just training hard and keeping my head down.
"There are a few clubs interested but nothing has materialised, then this rumour about me playing for QPR came out of the blue."
O'Hara has admitted he can understand why he became a target for some supporters as Wolves suffered back-to-back relegations and accepts some of his performances were below-par.
"A lot of things I've said have been misconstrued, such as people reading into it that I said I'm a Premier League player," he added.
"I never said I wanted to leave Wolves, what I said was I would love to play in the Premier League again one day if I got the chance.
"It's sad really but now, it seems, whatever I say is turned into a negative. The Wolves fans are frustrated by the performances on the pitch and what's happened over the last two years.
"My performances weren't good enough and I can understand the fans' frustrations but to blame one player is probably a bit harsh.
"But these things happen in football. The club paid good money for me and they expected big things of me.
"In some games I didn't play to the best of my ability and things didn't go my way, but I always gave 100 per cent."