A statement on the FA website read: "Club England medical staff have been in constant contact with their counterparts at Liverpool Football Club since he returned there on Thursday.
"His injury has not progressed as had been hoped and it is now clear that Sturridge will not be fit for the match in Kiev on Tuesday evening."
Although the news will not be a surprise to Roy Hodgson, the manager would have been quietly hoping the on-song forward would recover after aggravating a groin injury in Liverpool's triumph over Manchester United last weekend.
The 24-year-old also missed England's 4-0 win on Friday night against Moldova, but injuries to Manchester United's Wayne Rooney and West Ham's Andy Carroll, plus the suspension of Danny Welbeck, leaves England with a selection headache.
With two goals in his first two England games, it now seems that Southampton's Rickie Lambert will be tasked with leading the line in Kiev - with Tottenham's Jermain Defoe the only other fit striker in the squad.
The FA confirmed that Hodgson will not be drafting in any replacements to the squad of 23, who are due to travel on Sunday evening.
With England leading Group H, the pressure is on Ukraine to go for the spoils and they will be confident of upsetting the visitors having smashed nine goals past San Marino on Friday.