There has always been a bitter rivalry both on and off the pitch when the two sides meet but matters boiled over at White Hart Lane last season.
Some Arsenal supporters directed abuse at Emmanuel Adebayor while a section of Tottenham fans were heard chanting offensive songs at Arsene Wenger.
A statement published on the Arsenal and Spurs websites read: "Both clubs are actively encouraging all fans to focus on getting behind their team and enjoying what promises to be a great match on Saturday.
"A north London derby is always a special occasion and we hope Saturday's match will be remembered for both the action on the pitch, as well as the positive support for both teams off it.
"All fans should be aware that breaches of stadium regulations - including anti-social behaviour - will not be tolerated.
"Arsenal and Tottenham do not tolerate discrimination of any sort, whether it be on the pitch or in the stands.
"Both clubs are actively encouraging all fans to focus on getting behind their team and enjoying what promises to be a great match on Saturday."
Tottenham have also warned their fans that any offensive banners will be confiscated upon entry to the stadium.