Arsenal have failed with two club record bids for Suarez, who told Liverpool earlier this summer he wants out of the club but has since returned to pre-season training.
And as Sky Sports News' 92Live helicopter landed in Liverpool on Thursday evening, Ayre joined Jim White pitchside at Anfield to make it clear the striker is going nowhere before the transfer window closes.
"We've got Luis Suarez in our squad, he'll have trained today and he's working hard," said Ayre, who added there have been no offers from abroad for the player.
"Despite what people think we've not had an intention of selling Luis, we've never said we want to sell Luis.
"He continues to work hard and train hard for Liverpool. That will continue and I'll expect him to be here at the start of the season."
Ayre confirmed Arsenal's two bids but said: "It's not something we're interested in and, as our fans would expect, we rejected both. The player is not available for sale - that's where we are and that's where we'll stay
"I wish I had £1 for everyone that's stopped me in the street saying 'make sure Luis stays'. That's what we're doing."
Suarez was given time off after playing for Uruguay in the Confederations Cup but joined up with Brendan Rodgers' squad in Melbourne, where 95,000 supporters watched Liverpool beat Melbourne Victory.
Ayre said: "I don't think his demeanour's changed - he's been a professional throughout. It's just business as usual.
"Luis was on the back end of tour with us. He joined us in Melbourne, and seeing the fantastic support and adulation, nobody will know more than Luis how much he's loved by this football club.
"I'm sure he's going to stay - that's always been our ambtion and that's what we expect to achieve."