The Latics outplayed champions Manchester City for long spells on Wednesday only to succumb to a lone Carlos Tevez stunner, ensuring they remain three points adrift of safety, albeit with a game in hand.
Martinez's men have staged two successive late-season recoveries but the Spaniard is braced for their toughest challenge yet.
Martinez said: "If you watch our performance and think Wigan Athletic are a team in the bottom three you would scratch your head. That brings disappointment.
"We have been in this position before and know what to expect but that doesn't make it easier.
"It is going to be a really tough ending of the season and for me it is the hardest battle we have had for a few years but we are ready for it.
"The performance was as good as it gets but sometimes getting the three points is out of your hands and that was the case.
"We have got things very clear. We need to perform exactly the same as we did here. If we do that the points will come, there are no two ways about it."
Wigan have six more league games left to play, starting with this weekend's trip to West Ham, as well as an FA Cup final reunion with City.
"It is a really exciting period with an FA Cup final right in the middle of it," Martinez added.
"The team is fresh, excited and if we play like last night we will get a lot of points and good moments.
"We don't look at the table. That is something you maybe hear too often but it is the truth.
"We didn't go to Manchester City for a point or damage limitation. We wanted to win and we deserved to win. But we have to learn. When we deserve to win, we have to win."