The 29-year-old centre-back was all set to complete a transfer to Ole Gunnar Solskjaer's men, who had met the Latics' asking price, before the deal fell through with injury concerns appearing to dissuade the Bluebirds.
Ramis has featured just seven times in 13 months due to a knee injury and a soft-tissue problem but has now returned to full training and could make his comeback in the fifth round at the Cardiff City Stadium.
Like boss Uwe Rosler and many of the current Wigan squad, the Spaniard played no part in the glorious FA Cup campaign which culminated in a memorable May victory over Manchester City at Wembley, but the manager suggested Ramis could draw upon another source of inspiration.
"Who wants to play against Cardiff? One of them desperately wants to play to prove them wrong and it's not Nicky Maynard because he is cup-tied," said Rosler.
The holders will travel in high spirits after Tuesday's 3-0 win at Sheffield Wednesday emphatically ended a poor sequence of away performances which had seen them score just five in the 13 games since a 4-0 thrashing of Barnsley on the opening day.
Rosler has inherited possibly the busiest schedule in English football after assuming the helm in early December with Wigan due to play their 41st game of a season which has included jaunts to Russia and Slovenia.
"I have a few options," the German noted.
"The game (against Sheffield Wednesday) took a lot out of them, we will bring fresh legs in. Everybody will be trying to get into the last eight, that's our aim.
"Cardiff have some very important games coming up, like we do. We will utilise the squad. We are going there and have a 50-50 chance. I wouldn't say we are complete outsiders but everybody has to have a good day."
One man who Rosler will not be able to call upon is Maynard, borrowed from the Bluebirds until the end of the season.
He opened his account with a brace against Wednesday - although the second goal is a bone of contention as Martyn Waghorn also staked a claim - with Rosler hopeful he can finally give his attack a much-needed spearhead.
However, the Latics boss insists any post-match conversations with Solskjaer are unlikely to revolve around making Maynard's move permanent.
"To speak to about permanent things is too early," Rosler said.
"I enjoy working with him and I know he wants to do everything to prove me right because I said to the chairman (Dave Whelan) that Nicky Maynard can be very important for us in terms of giving us the goals we need."