Peltier turned his ankle towards the end of the weekend defeat at Charlton and is waiting for a full prognosis as Leeds look to avoid a fifth straight defeat and halt their slide towards the relegation zone with victory over Yorkshire rivals Wednesday.
Fellow central defender Tom Lees will sit out the final game of his three-match ban and Redfearn could be left short at the heart of defence.
"He went over on his ankle," Redfearn told the club's official website.
"There was a lot of swelling immediately afterwards so it was iced and it was a case of waiting for the swelling to go down before an assessment can be made.
"It didn't look good, but it's very difficult to judge or comment on until he can be assessed properly."