Sunderland go to Carrow Road in the bottom three but with at least two games in hand on all their relegation rivals.
The first of those is against a Norwich side just four points ahead and, whatever happens between now and May, Poyet wants his players to emerge from the run-in with no regrets.
He said: "I think this is a crucial game for both teams. Every single game is crucial.
"The idea is to take every game as the last one - a cup final, people call it - you need to win this one to stay up.
"There are 10 teams at the bottom, If every single player gave everything from now to the end of the season, still three teams will go down.
"So the main thing is to be yourself, play the way you play, accept responsibility and go and give everything. Then, there are no regrets."
Sunderland are without a league win since beating Newcastle 3-0 in the north-east derby on February 1.