Coach Sean McCarthy and scouts David Hamilton and Mark Seagraves have left Portman Road as new manager Mick McCarthy begins to restructure his support team.
Former Oldham striker McCarthy joined the Ipswich coaching staff under previous manager Paul Jewell in January last year, and Town boss McCarthy admitted it was a tough decision to let him go.
"Sean has been brilliant for me and Terry Connor since we've been here and helped us settle in but I don't need another coach," said the former Wolves manager.
"I also want to bring my own staff in as well - so Sean has left the club. He is a football man, he understands how it works. There is no animosity at all in his departure and he goes with my best wishes for the future."
McCarthy added: "Ipswich is a great club and it's been an honour to work here. When you work in football though, you know there will inevitably be changes when a new manager comes in. That's part of the game.
"I have enjoyed working with Mick over the last few weeks. He has been first class with me and I hope he can turn things around here. I wish everyone at the Club the best of luck."
Ian Evans and Dave Bowman are poised to be added to the scouting set-up along with Steve McCall.