The reverse left the Canaries six points above the Premier League relegation zone with three matches remaining and Hughton concedes it is a "nervy time".
Since mid-December, Norwich have won just two league games and their demise arguably traces back to a 4-1 Capital One Cup loss to Villa at Carrow Road that ended a 10-game run without defeat.
Hughton, however, is adamant that match will not be a factor for the return visit of Villa and former Norwich boss Paul Lambert.
"It's a massive game for us because of the fact we are playing a team around us, so there is a little more significance in the game," said Hughton.
"As for the game back in December, I don't think it has any bearing on the one coming up.
"You might have seen the away game (a 1-1 draw at Villa in October). I don't think there is anybody who saw that who didn't think we were the better team on that day, so the cup game is irrelevant.
"Regardless, it's a tough game at this stage of the season whoever it is against."