The game was marred by supporters amongst the Millwall contingent attacking police and fighting among themselves inside and outside London's Wembley Stadium.
Fourteen people were arrested on the day, April 13, police said - 12 Millwall supporters and two Wigan fans.
Officers from Operation Arrowtip - Scotland Yard's response to football-related crime - want to identify everyone involved in the trouble and appealed for anyone with information to help.
Detective Chief Inspector Andy Barnes said: "We want to reassure the public that we are working closely with our partners to do everything possible to remove these people from the stands.
"We are committed to bringing them to justice and in doing so we will seek to obtain lengthy football banning orders for all those found guilty at court.
"Over 60,000 fans went to this game to support their teams, sadly there was a small minority intent on violence. In sending a clear message to those intent on causing trouble, I would appeal for help from the public to identify any of the suspects featured in the images we are releasing today."
Wigan won the match 2-0 to set up a Wembley final against Manchester City next month.