The Italian side's physical approach to defending at corners and free kicks enraged the Celtic players in the tie's first leg, which saw the Scots crash to a 3-0 defeat.
Juventus went unpunished in Glasgow, and Celtic's Greek striker wants tonight's referee, Firat Aydinus, to stamp his authority on the game.
"It wasn't fair, I think everybody saw that," said Samaras, who missed the first leg. "It is how they defend set-pieces, like most Italian teams.
"Hopefully, the referee will have his eyes more open tomorrow and see clearly what's going on and it can be a fair game."
The heavy defeat at Parkhead leaves Celtic with a daunting task, but Samaras retains slim hopes of an unlikely comeback.
"We will play the same way as we did in the first leg, try to move the ball, play attacking football and create chances.
"If we score the first goal you never know. In football everything can happen."
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