Ranieri's side are unbeaten in Ligue 1 this season and they will be looking to take that form into the meeting with fellow top-flight side Reims at the Stade Auguste-Delaune.
The Monaco boss confirmed that he will hand a debut to goalkeeper Sergio Romero, who joined on loan from Sampdoria this August, along with making a few other changes to his starting line-up.
The Italian challenged the incoming players to make an impression as Monaco bid to improve on their Coupe de la Ligue run last year, where they suffered a 2-1 home defeat to Troyes in the round of 16.
"On Wednesday night, the players will have the opportunity to show their quality," Ranieri told a press conference.
"I want to think exclusively (about the game) in Reims.
"Sergio Romero will play in the cup game on Wednesday and we must keep up this good momentum even with a changed starting line-up."
Elsewhere in the round of 32, Guingamp take on Evian in an all-Ligue 1 clash, while Lille face a tricky tie against second-tier Auxerre - who have already defeated Brest and Caen - on Tuesday.
Ligue 1's Valenciennes will look to bounce back from a 1-0 home league defeat by Evian when they face Ligue 2 opposition in the form of Troyes, while Rennes will also take on a second tier side as they host Nancy.
Toulouse travel to the Stade Dominique Duvauchelle to take on Creteil while Montpellier make the trip to top-flight strugglers Sochaux.
There is one match which ensures there will definitely be representation from outside of the top division, as Ligue 2's Tours host Championship National side Amiens.
In the other games, Bastia will bid to overcome Ajaccio and Nantes will aim to move on from their 1-0 league defeat by Lille by targeting victory over Lorient.