The World Player of the Year missed training and needed treatment on Friday after coming off in Thursday's King's Cup quarter-final second leg win at Malaga.
But Messi took a full part in training with the rest of the squad on Saturday and is fit to feature at home against Osasuna on Sunday evening.
A Barca statement on the club website said: "The Argentine forward (Messi) will be available for selection for the next league fixture."
The news will come as a boost for Barcelona, who also have Alex Song back in the squad, as they look to return to winning ways after losing their unbeaten start to the season against Real Sociedad last weekend.
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