Roy Hodgson and his Three Lions squad were handed a tough draw in Salvador in Brazil on Friday, with a trip to the sweltering Amazon region first up on 14 June (kick-off 2am UK time on Sunday, 15 June).
Following that clash with the Azzurri, England must take on top seeds Uruguay, featuring Liverpool star Luis Suarez, in Sao Paulo on Thursday, 19 June (8pm UK time) before ending their group-stage challenge against Costa Rica in Belo Horizonte on Tuesday, 24 June (5pm UK time).
Hosts Brazil will face Croatia in the opening game of the tournament in Sao Paulo on Thursday, 12 June.
And in a replay of the 2010 final, Spain face the Netherlands in a Group B clash in Salvador the next day.
Other stand-out games include Lionel Messi's Argentina against debutants Bosnia in a Group F opener in Rio de Janeiro on Sunday, 15 June; a clash between heavyweights Germany and Portugal in Salvador the following day; and another Group G encounter involving Germany in Recife on Friday, 20 June, when Jurgen Klinsmann's United States will provide the opposition.
Italy were the European team drawn at random to join Pot Two ahead of the main group-stage draw.
FIFA decided in advance of Friday's ceremony that one of the nine unseeded European teams for the finals would be put into the pot containing the unseeded South American and African countries.
That meant Italy were guaranteed to face a seeded South American side - and were promptly placed with Uruguay in Group D, with Costa Rica and then England joining them.
The FA have confirmed England will still base themselves in Rio de Janeiro for the tournament.
Manaus is a five-and-a-half hour flight from Rio and is located in the Amazonian rainforest, with 99% humidity.
The one crumb of comfort is that England face a European side in such a remote and unusual location, 1,777 miles from where they will be staying.
Sao Paolo and Belo Horizonte are much more accessible from Rio. It is just over 200 miles to Sao Paolo and less than 300 to Belo Horizonte.
However, that place has unhappy memories for England as the venue where they lost to the United States in one of the biggest World Cup shocks of all time in 1950.
With regards to the draw, Hodgson told Sky Sports News: "In 2010, everyone was writing what an easy group England had and predicted that they would sail through but it didn't happen. Each game was tough.
"Having a group people say is tough can, in someways, be an advantage.
"Don't forget we've unearthed some good players in the last few years and rejuvenated the squad.
"It is definitely a lot stronger and deeper, and hopefully we won't get too many injuries before then."
Speaking about Uruguay and their star striker Luis Suarez of Liverpool, Hodgson added: "We know what a good player he is, trying to put a spoke in England's wheels.
"But we have good players too. Maybe we'll all be talking about Wayne Rooney and Daniel Sturridge when the tournament is over.
"We know less about Costa Rica but they are a good team too."
A: Brazil, Croatia, Mexico, Cameroon
B: Spain, Netherlands, Chile, Australia
C: Colombia, Greece, Ivory Coast, Japan
D: Uruguay, Costa Rica, England, Italy
E: Switzerland, Ecuador, France, Honduras
F: Argentina, Bosnia-Herzegovina, Iran, Nigeria
G: Germany, Portugal, Ghana, United States
H: Belgium, Algeria, Russia, Korea Republic