Former West Brom forward Fortune has been used as a substitute in all four of Latics' fixtures to date with fellow new signing Holt preferred in Coyle's solitary striker system.
However, two goals down to Rovers on Tuesday night, Fortune came on at half-time to join Holt up top and had a part to play in both goals as Wigan claimed a point in Coyle's first home game at the helm.
The manager said: "Fortune is a top player and Marc has probably been a little bit unlucky because he had a very good pre-season and obviously the way we set up with the three in the middle with the one main striker then Holty just got the nod.
"But we know Marc-Antoine Fortune can play; he's a top player and he's certainly banging on the door for that starting spot."
When pressed on whether he would dispense with a midfielder in order to accommodate both Fortune and Holt in his XI, Coyle added: "That's something you always look at.
"It's something we'll give good thought to and we have to pick a team that we feel can beat Middlesbrough at home and that's what we'll look to do.
"As a former striker myself I'm always on that we should be more clinical so we'll keep stressing that as well. Hopefully if we create more chances, we'll score more goals."