The legends match, which kicks off at 2pm on the Bank Holiday Monday, will feature some of the finest players to turn out for both clubs.
The Rangers Charity Foundation and UNICEF, the world's leading children's organisation, will both receive donations from the proceeds of the game and a gala dinner later that evening.
The Rangers legends side, managed by Walter Smith and captained by Richard Gough, features a number of players from the nine-in-a-row era of the late 80s and early 90s.
Among those turning out will be present boss Ally McCoist, Brian Laudrup, Arthur Numan, Lorenzo Amoruso, Michael Mols, Jorg Albertz, Gordon Durie, Alex Rae and Marvin Andrews.
United, managed by former Old Trafford hero Bryan Robson, will field a team which includes Edwin Van Der Sar, Denis Irwin, Dwight Yorke, Quinton Fortune, Jesper Blomqvist and David May.
Commenting on the match McCoist told the club's official website: "This is a fantastic game to look forward to at the end of the season and I know our supporters will turn out in huge numbers as they did for the AC Milan Glorie match last year.
"It was a pleasure to pull on the boots again at Ibrox that night and I am looking forward to doing so again against Manchester United's legends.
"I am sure it will be another special afternoon at Ibrox as I will be playing with lifelong friends again and the fact the Rangers Charity Foundation and UNICEF will both benefit adds to the occasion."
David Bull, executive director of UNICEF UK said: "We are thrilled that two of the world's most well-known football clubs, both long standing supporters of UNICEF are coming together in support of the world's children.
"Every day, more than 20,000 children under the age of five die from preventable diseases. The funds raised for UNICEF at this event will go towards providing life saving vaccines for children across the globe."