The Dons looked set for a battling point at Cheltenham on Saturday until Jamie Cureton struck deep into stoppage time to snatch a 1-0 win for the hosts.
"They were gutted," said Ardley. "It is now a case of the staff and I picking them up off the floor and us bouncing back, something we have done so far this season.
"We need to come back from this and produce good performances and pick up points."
Meanwhile, defender Will Antwi will build up his match fitness with a run-out for the club's Under-21s on Tuesday night.
Antwi has yet to play this season following a hernia operation.