Chelsea left-back Patrick van Aanholt, who has spent the season on loan at Vitesse Arnhem, is the sole Premier League representative in the 23-man party heading to Israel in June.
Among the ones to watch in the Dutch squad is highly-rated PSV Eindhoven midfielder Kevin Strootman, who was with the Oranje at Euro 2012 and has reportedly been tracked by Manchester United and a host of other top European clubs.
Left-back Daley Blind, the son of former Holland and Ajax left-back Danny, is also included, while FC Twente's Leroy Fer came close to joining Everton in January only to see the transfer fall through.
Holland are up against Germany, Spain and Russia in Group B at the Under-21 Euros, which get underway on 5 June.
Netherlands Under-21 squad:
Goalkeepers: Marco Bizot (Groningen), Nick Marsman (FC Twente), Jeroen Zoet (PSV Eindhoven).
Defenders: Patrick van Aanholt (Chelsea), Daley Blind (Ajax), Mike van der Hoorn (FC Utrecht), Kelvin Leerdam (Feyenoord), Bruno Martins Indi (Feyenoord), Bram Nuytinck (Anderlecht), Ricardo van Rhijn (Ajax), Stefan de Vrij (Feyenoord).
Midfielders: Jordy Clasie (Feyenoord), Leroy Fer (FC Twente), Marco van Ginkel (Vitesse), Adam Maher (AZ Alkmaar), Kevin Strootman (PSV Eindhoven), Tonny Vilhena (Feyenoord).
Forwards: Memphis Depay (PSV Eindhoven), Ola John (Benfica), Luuk de Jong (Borussia Monchengladbach), Florian Jozefzoon (RKC Waalwijk), Jurgen Locadia (PSV Eindhoven), Georginio Wijnaldum (PSV Eindhoven).