An Alderney registered Irish-based betting website contacted the Daily Telegraph and claimed that he had identified two matches since 2000 that showed signs of having been manipulated.
The betting exchange operator, which specialises in Asian handicaps, alleges that he was "99 per cent certain one game was fixed".
In response the Premier League's chief executive Richard Scudamore urged anyone with who had any allegations of match fixing to contact the police
"This is an allegation of criminal acts. It is the most serious allegation that can be made about anyone involved in sport. It is about the most serious attack that can be made on sport," said Scudamore.
"Anyone with allegations, information or evidence should report it to the Police. There are processes in place through which they, the Gambling Commission and the Football Association are to act following receipt of such information.
"We would of course assist any such investigation."