Tottenham striker Jermain Defoe and Manchester United midfielder Michael Carrick will miss England's friendly against Brazil on Wednesday while Daniel Sturridge is also doubtful.
Spurs manager Andre Villas-Boas confirmed after Spurs' 1-0 win at West Brom that Defoe would be withdrawn from Roy Hodgson's squad ahead of the Wembley clash.
Villas Boas said: "I am extremely happy with the win - apart from the injury to Jermain Defoe.
"He rolled his ankle. He will have to have a scan to reveal the extent of the injury.
"Hopefully there is not a lot of ligament damage. If there is, he could be out for two to three weeks.
"Has he been withdrawn from the England squad? Yes he has."
A statement on the FA's official website said Carrick is suffering from a hip injury and will not join Roy Hodgson's squad.
Liverpool striker Sturridge is a doubt after sustaining a thigh injury in the 2-2 draw at Manchester City.
The 23-year-old, who has just four senior caps, scored a brilliant goal at the Etihad Stadium to make it 1-1 in the first half but then picked up a knock.
He remained on the pitch until he was substituted just before the final whistle but Rodgers is not optimistic about his chances of joining up with England.
"He got a knock just before half-time on his thigh and he wanted to give it a go in the second half," said the Reds boss.
"I think he will be a doubt for the game on Wednesday, which is unfortunate because he was terrific today.
"His hold-up play, movement and pace was a real threat, and combination with Luis Suarez, Stewart Downing and Jordan Henderson up front was outstanding.
"He is just with the medics at the moment and he will have a chat with our doctors and he will speak with the England set-up.
"They might want to have a look at it but at the moment he is obviously struggling."
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