Portsmouth suffered a first-round FA Cup exit for the second successive year as the club that triumphed in the competition at Wembley in 2008 slumped to defeat at Stevenage.
Last year it was Notts County reminding Portsmouth of how far they have fallen in such a short time, claiming their scalp. On this occasion, Stevenage were two goals up and had a man advantage before Portsmouth made a game of it at Broadhall Way.
Stevenage took a 2-0 lead with a pair of goals from Francois Zoko in the first half, and Gavin Mahon's dismissal early in the second half looked to kill Portsmouth's hopes.
They narrowed their deficit when David Connolly made it 2-1 in the 71st minute, but the scoreline stayed that way as League One strugglers Stevenage clinched victory over their League Two opponents.
Amy Fearn made FA Cup history as she became the first woman to referee a match in the main draw of the competition, overseeing Dover's 2-1 victory at Corby.
The 35-year-old, who has refereed in the Football League, had a satisfyingly uneventful game as Dover won 2-1 in Northamptonshire with goals from Nathan Elder and Chris Kinnear. Corby's late reply came from Chris Carruthers.
When St Albans of the Southern League Premier Division went 1-0 up against Sky Bet League Two side Mansfield, a cup shock looked a possibility. Come full-time, however, St Albans were nursing their wounds after taking an 8-1 drubbing, with Sam Clucas scoring four times in the final 20 minutes, the magic of the cup having well and truly faded.
Conference North outfit Brackley went closer as they faced Gillingham, taking the lead at Priestfield Stadium with a Joe Martin own goal in the 69th minute but conceding a stoppage-time leveller to home substitute Bradley Dack that will force a replay.
Sutton United have their place in FA Cup history locked up, having knocked out then holders Coventry in the third round 25 years ago, but there will be no repeat of their 1989 heroics this time after a 4-1 thrashing at Kidderminster.
Sutton briefly led through Charlie Clough but Amari Morgan Smith, Callum Gittings, Joe Lolley and Anthony Malbon scored to inflict a heavy defeat.
Conference side Macclesfield caused a major surprise by beating League One Swindon 4-0, thanks to two goals from Connor Jennings and one each for Scott Boden and Peter Winn.
Oxford, second in League Two, almost tripped up at home to Conference side Gateshead, who took a two-goal lead through James Marwood and Jamie Chandler. The hosts forced a 2-2 draw and a replay with late replies from Deane Smalley and Danny Rose.
Former Holland midfielder Edgar Davids fielded himself as a striker for Barnet against Preston, but it was far from a masterstroke from the 40-year-old player-manager, whose Conference team lost 6-0 at Deepdale, where Paul Gallagher scored a hat-trick and Joe Garner a double. Both teams had a man sent off.
Davids later wrote on Twitter: "Today a massive spanking but we don't forget the lesson."
Conference Premier strugglers Tamworth caused a surprise by overcoming Cheltenham of League Two 1-0, with the only goal at the Lamb Ground coming in the 20th minute from Nick Chadwick.
Conference South side Boreham Wood and Conference Premier outfit Braintree earned replays after home draws against Football League opponents, Carlisle and Newport respectively.
But many non-league teams found the going difficult, with Staines Town brushed aside 5-0 at Brentford, Southport beaten 5-2 by League One leaders Leyton Orient, and the re-born FC Halifax losing 4-1 away to MK Dons.
Daventry Town, from the Calor League Division One Central, gamely battled at Chesterfield for over an hour before eventually succumbing 2-0 to their League Two opposition. Gary Roberts broke the deadlock after 69 minutes and Jimmy Ryan added a late second.
Former Football League sides Grimsby and Lincoln earned replays after goalless home draws with Scunthorpe and Plymouth respectively.
Four ties paired non-league sides together, with Dan Fitchett netting twice as Salisbury beating Dartford 4-2, Ryan Rowe with a double in Stourbridge's 4-1 win against Biggleswade Town, Welling toppling Luton 2-1, and Wrexham ousting Alfreton 3-1.
Gloucester's hopes were ended by a 2-0 home defeat to Fleetwood, while Hednesford bowed out 2-1 to Crawley.
Among all-Football League games, Tranmere beat Accrington 1-0, Bristol City turned over Dagenham & Redbridge 3-0, and Sheffield United came out 3-2 winners at Colchester, the same scoreline that favoured Hartlepool in their home match against Notts County.
Southend, Peterborough, Rotherham, Rochdale and Walsall all marched on, as Morecambe, Exeter, Bradford, Torquay and Shrewsbury saw their hopes ended.
Oldham and Wolves will replay, as will Wycombe and Crewe.