Arsenal midfielder Jack Wilshere faces six weeks on the sidelines after suffering a hairline fracture in his left foot during England's friendly international with Denmark.
Wilshere returned to the field after treatment and played on until the 59th minute, revealing afterwards he suffered no ill effects from the challenge.
However, Arsenal followed-up the Football Association's initial scans on Thursday and found a fracture which will see the midfielder miss the next six weeks.
"Jack Wilshere has sustained a hairline fracture to the navicular bone in his left foot," a joint statement by Arsenal and the FA said.
"Wilshere suffered the injury during England's international match with Denmark on Wednesday night at Wembley Stadium, in which he played 59 minutes.
"Following initial scans carried out by the FA on Wednesday night, further investigation by Arsenal's medical team has discovered a crack to a small bone in Jack's left foot - the navicular, an area of his foot which has not been previously injured.
"Jack will now require a period of approximately six weeks' rehabilitation, before starting to regain his match fitness."
The injury is a huge blow to Arsenal, whose crucial run-in to the season continues with an FA Cup quarter-final against Everton on Saturday.
The news will no doubt have been a huge surprise to Wilshere, who confidently told reporters after the match that he was fine before speaking of his desire to finish the season strong with Arsenal.
"It is a bit sore, but just a bruise," he said. "I have had a scan already and I will be all right.
"We have all been working hard in training trying to impress the manager, but at the end of the day, it is your club form which will get you in.
"These next two months are crucial, it is a big time of the season and then when the England squad is announced, you can only perform to the best of your ability."
The prognosis means Wilshere will miss at least eight Arsenal matches, before making his Premier League return in the trip to Hull on April 19.
As well as harming the Gunners, that will give the midfielder little time to get himself match fit before Roy Hodgson names his World Cup squad on May 13.
The England boss spoke after the match of his hopes that nobody would join Theo Walcott in missing the tournament and struck a relieved figure after Wilshere appeared to avoid serious injury.
"The last thing I want is to send players back injured especially when Arsenal have so many important games coming up, so we were delighted when he got up and we were told it was just a kick on the foot," the England manager said.
"I am hoping that when he goes back to Arsenal they will soon put that right and that he will be fit for Saturday."
The fracture was so miniscule that the scanning equipment within Wembley failed to detect it when Wilshere left the pitch.
It is understood the England medical team only decided to scan Wilshere's foot as a precaution and there was no indication from the results that the player had suffered a fracture.
It was only when Arsenal conducted a more detailed examination of the injured area that the real problem became more apparent.
Although it will be of little comfort to the club, Arsenal will be compensated for the injury.
The Gunners will not have to pay for the midfielder's wages while he is out.
On the assumption that Wilshere is out for six weeks and earns £100,000 a week, Arsenal will pocket £600,000.
That money will not come from the FA, but from the organisation's insurers.
The FA is part of an insurance scheme that protects it from shelling out huge sums to clubs in the event that a player becomes injured on international duty.
The FA is one of the few national associations who run such an insurance scheme.