Midfielder Gleeson has been used at right-back by manager Karl Robinson this season but was replaced during Saturday’s 4-1 hammering at Carlisle with a muscle problem and is a doubt to face Ian Holloway’s men.
Mat Doumbe is an option to start if Gleeson misses out, while Robinson may also make changes.
Dietmar Hamann will be rested and Mark Carrington, Lewis Guy, Jabo Ibehre, Luke Howell and Adam Chicksen will all hope to be given a chance to start.
Defender Gary Mackenzie is still waiting for his debut as he recovers from an injury picked up in the summer while striker Aaron Wilbraham is out with a back problem.
Jude Stirling also misses out as he rests his groin injury and Pele begins a lay-off after pulling a thigh muscle.