Earlier this year the association's chief executive, Gordon Taylor, was found not to have breached any rules, despite amassing gambling debts.
But Carlisle, who will soon be succeeded by Ritchie Humphreys, wants to prevent similar incidents in the future by enforcing a betting ban on all employees.
He told Sky Sports News: "What we need to do for our members is ensure that we make them aware of the potential pitfalls, of the possibilities of getting into dark waters in this issue.
"But I think more prevalent for us as an organisation is that we need to make sure that we maintain the integrity of the sport within our behaviours.
"In light of recent conversations and recent events, we're going to discuss the protocol and behaviours for actual PFA employees.
"Not our members, actual employees of the organisation because to all intents and purposes we do have access to the entire member base of football and to safeguard us from any questions of irresponsible behaviour or bringing the game into disrepute, we're going to take steps to ensure that doesn't happen."