The new regulations ban football club employees with teams in the top eight levels of English football from placing bets on any matches and leagues worldwide.
Previous rules only banned players from betting on competitions their teams are participating in, with Premier League players Dan Gosling and Andros Townsend among those to face disciplinary action for breaches.
On top of that, the game has been hit by a number of match-fixing scandals with former Premier League player Sam Sodje among those arrested during investigations into corruption within the sport.
The FA has released a film to clubs to explain the new rules, and general secretary Alex Horne says they will take away any grey areas.
Horne told Sky Sports News: "What's interesting is that these rules are easier to police so quite simply, if you play above Step Four (Isthmian League, Southern League, Northern League) you don't bet on football.
"It's a much easier message to convey, to communicate and to understand if you are a player, a coach, a manager or a referee.
"The previous rules were possibly a little bit open to interpretation because it was around the competitions that your club played in.
"It's a simple rule. It isn't because we believe we have a cultural problem or an issue but it's a perceptive issue betting on football by people playing in football.
"It's just part of our war on match-fixing. Integrity of the game in England is crucial for us and it's what makes the English game so exceptional. It is completely trustworthy and we want to do everything we can to keep match-fixing out of our game."