Dolan has been talked of as a potential replacement for Brian McDermott at Elland Road, with Leeds owner Massimo Cellino seeking a domestic head coach to take over.
But Reading director of football Nicky Hammond revealed the club has no intention of letting Dolan leave the role he has been in since leaving Exeter City 10 years ago.
Hammond said: "Reading FC are aware of the interest of another Championship club in our academy manager Eamonn Dolan.
"Eamonn is an integral part of our club and is under contract to Reading Football Club.
"Eamonn has done an incredible job since taking over from Brendan Rodgers and we hope he will be a big part of our club for many years to come.
"He has a fantastic relationship with our manager Nigel Adkins and we do not welcome any approaches from any club for our key staff. We hope this is the end of the matter."