Blackburn defender Grant Hanley has withdrawn from Strachan's squad ahead of Wednesday's match in the National Stadium in Warsaw.
Norwich winger Robert Snodgrass already pulled out ahead of his team's 4-1 defeat by Aston Villa earlier on Sunday, because of a recurring muscle problem.
The Scottish Football Association announced on Saturday that Celtic striker Leigh Griffiths would replace Snodgrass.
Griffiths may find himself in the starting team following his hat-trick for the Scottish champions in the 5-0 thrashing of Inverness on Saturday.
Speaking after that match on the possibility of winning his fifth cap, Griffiths said: "It is a bonus. I am absolutely buzzing to go."