Holders Kilmarnock were knocked out of the Scottish Communities League Cup in the second round on Tuesday night while fellow SPL sides Hibernian and Ross County also suffered shock defeats.
Kilmarnock, who beat Celtic 1-0 in last season's final, were beaten 2-1 by Second Division side Stenhousemuir at Rugby Park.
Stenhousemuir hit two first-half goals to maintain their unbeaten run this season and dump Kilmarnock out of the Scottish Communities League Cup at Rugby Park.
Brown Ferguson scored a penalty to put the underdogs in the lead before John Gemmell's close-range strike shortly before half-time doubled their advantage.
The SPL side threw everything at Stenhousemuir after the break and nearly forced extra-time when Michael Nelson's last-gasp header found the net before a frantic goal-mouth scramble ended up in the hands of goalkeeper Calum Reidford.
Hibernian were also sent crashing after a 2-0 defeat at Second Division side Queen of the South.
Nicky Clark, son of former Hearts striker and current Queens assistant Sandy Clark, put the Dumfries side on their way to a thoroughly-deserved victory with a close-range finish on 12 minutes.
Substitute Gavin Reilly added a second with a diving header in the 42nd minute and the home side continued to look the more enterprising team.
Raith Rovers caused a major upset against Ross County with a 4-1 victory at Global Energy Stadium.
Brian Graham netted in either half and Greig Spence and Dougie Hill got the others as the Division One outfit ran County's makeshift side ragged.
A late Russell Duncan goal was all the home side could muster on a night to forget for the SPL new boys.