The 24-year-old rounded off an impressive first season at Real Madrid by heading the crucial goal in Saturday's 4-1 Champions League final victory over rivals Atletico Madrid.
Following the celebrations, Bale had been due to join up with Chris Coleman's squad ahead of putting World Cup hopefuls Holland to the test in Amsterdam on Wednesday.
But a statement from the Football Association of Wales said: "Gareth Bale has withdrawn from the Welsh squad to travel to Amsterdam to face the Netherlands in a friendly international next week.
"Bale, who scored for Real in Saturday's Champions League final win against Atletico, has been troubled with a leg muscle injury for a few weeks.
"His place in the squad will be taken by Fulham's George Williams."