The Bees were missing Wojciech Szczesny, Pim Balkestein and James Wilson for Saturday’s 1-0 FA Cup victory over Walsall but all three will be in the Bees’ squad.
On-loan duo Szczesny and Balkestein were refused permission by their parent clubs to play against the Saddlers, while Wilson served a one-match ban.
Myles Weston has recovered fully from a hamstring injury and will fight for a place in the matchday 18, while Charlie MacDonald overcame a groin injury to play at the weekend and should again start.
On-loan centre-back Leon Legge is guaranteed a starting berth after he scored the winning goal against Walsall and earned glowing praise from Scott.
The Bees welcome an impressive Colchester side to Griffin Park desperate to avert a poor run of form that has seen them go eight league games without a win, leaving them three points above the drop zone.