Colchester boss John Ward has hinted that he will make changes to his starting line-up for the game.
The U’s boss saw his side fall to their third defeat in four games with a 1-0 reverse at Dagenham on Tuesday night and he looks set to react by shuffling his place against the Dons.
“One or two areas in the team are up for grabs. I will be looking at training and we’ll come up with a team that can give MK Dons a good game,” Ward told the club’s official website.
Ward will again be without striker David Mooney, who serves the second game of a three-match ban, while defenders Brian Wilson and Morten Knudsen remain doubtful despite having returned to training following ankle and knee problems respectively.
Fellow defender Magnus Okuonghae will be out for the rest of the season after undergoing surgery on an ankle problem.
MK Dons manager Karl Robinson could have Angelo Balanta back in his squad to face Colchester.
On-loan QPR forward Balanta has not featured since suffering an ankle injury in November but played 50 minutes in a behind-closed-doors match on Wednesday and came through unscathed.
He could be on the bench and offers another attacking option for Robinson.
Midfielder Peter Leven completes a two-match suspension after picking up his 10th booking of the season during the 4-1 win at Rochdale last Saturday but central defender Sean O’Hanlon is back from a ban.
On-loan midfielder Keanu Marsh-Brown picked up an injury during the win over Charlton in midweek and is having his groin strain assessed by parent club Fulham.