League Two’s leading scorer registered his 21st of the season to see off Burton 1-0 at the weekend but was forced off in the 87th minute with a hamstring strain.
Le Fondre is desperate to face his former employers but Millers manager Ronnie Moore is keen to ensure he is 100% fit for the clash and is not prepared to risk losing his star man for any longer than necessary.
Midfielder Gary Roberts continues to be doubtful with a shoulder problem, while long-term absentees Dale Tonge, Paul Warne (both foot) and Andy Liddell (Achilles) remain sidelined.
Recent loan signing Josh Walker should start once again having made his debut at the Pirelli Stadium as the fifth-placed Millers look for a positive display at Spotland.
And while Le Fondre will be hoping to do the damage against his former club, Chris O’Grady will be looking to do the same to Rotherham having already bagged 18 league goals this term for Dale. On-loan Millers midfielder Jason Taylor is ineligible to feature for the hosts.