The right-back was forced off with a knock to his foot after just 21 minutes of Saturday’s 2-0 win over Hartlepool.
Peltier has a chance of being fit but if he fails to make it, Tom Clarke, who replaced him at Victoria Park, will again deputise.
Clark is otherwise expected to make no changes to his winning line-up in what he expects to be a hard-fought encounter.
Clark told the club’s official website: “If they can’t win games, they are not losing; they have taken seven points from a possible nine.
“Prenton Park is always a tough place to go, so we know we are in for a tough game.”