Preston claimed the lead in the 12th minute of Tuesday night’s League One clash at Prenton Park, with Graham Cummins heading in strongly following a well-placed free-kick by Barry Nicholson.
It took Tranmere just two minutes to equaliser, Enoch Showunmi heading home following good work by Ryan Brunt.
John Welsh was not far off target with a shot from range before Brunt wasted a big chance when set clear in the 25th minute.
Then, Thorsten Stuckmann in the North End goal just held onto a James Wallace strike. It required an excellent save by Stuckmann to deny Lucas Akins in the 54th minute, before another Wallace attempt was deflected for a 64th minute corner.
But Tranmere kept up the pressure and went ahead in the 67th minute when Andy Robinson fired in off a post after being set up by Ian Goodison. Preston kept battling away but rarely threatened home keeper Owain Fon Williams as they failed to win for a fifth match running.