Checkatrade Trophy holders Lincoln will face Wolves in the group stage of this season’s competition as they look to retain their crown.
As in the previous two seasons, there are 16 ‘invited’ teams competing alongside the 48 English Football League sides from League One and League Two.
The invited clubs come from those with category A academy status and must field under-21 sides, with Arsenal opting in to the competition for the first time.
In a slight tweak, only two players over the age of 21 who have more than 40 senior appearances can feature for invited clubs.
Wolves were drawn alongside Lincoln and well as Mansfield and Scunthorpe.
Manchester City will face beaten finalists Shrewsbury in northern Group D with fellow Premier League sides Leicester, Everton and Newcastle also in the northern half of the draw.
Arsenal will mark their debut in the Checkatrade Trophy in Group E of the southern section, coming up against Cheltenham, Coventry and Forest Green.
Chelsea reached the semi-finals last season and will face Newport, Plymouth and Swindon in Group C.
Tottenham, West Ham, Southampton, Fulham and Brighton are also involved.
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