The Viola have not lost a game since January 6 and are unbeaten in nine European away games, but Tottenham have not fallen at home in their last four European matches.
“It’s a great tie, a really tough game,” Tottenham’s Danish international midfielder Christian Eriksen told his club’s official website. “Fiorentina are doing well in Italy and they are a strong team.
“It’s always exciting in the knockout stage and it’s win or lose now – nothing in between. Everyone wants to go through and hopefully we’ll achieve that.”
Vincenzo Montella has done very well this season as Fiorentina coach.
His side has climbed to fourth in Serie A and also reached the semi-finals of the Coppa Italia, beating the club he once played for and later coached, Roma, along the way.
The Viola finished top of Group K to reach the last-32 of the Europa League.
Montella has impressed so much that he has been tipped as a possible coach for AC Milan. Right now, however, he is firmly focused on Tottenham.
“It’s a really difficult rival,” he said to the Italian media. “They have great players and they play a different style of football with respect to Serie A.
“It’s going to be very challenging but really stimulating for us.”
The teams have never met before in European competition.
Tottenham, who finished as Group C runners-up to Besiktas, go into the encounter after last week’s 3-2 defeat at Liverpool.
The outcome dropped Spurs to sixth in the Premier League standings, three points adrift of fourth-placed Southampton.
Mauricio Pochettino’s side have a Capital One Cup final to look forward to against Chelsea at the start of March.
Fiorentina’s on-loan winger from Chelsea, Mohamed Salah, is set to start for a second straight game after playing well in Saturday’s 3-1 triumph at Sassuolo.
Salah scored his first goal for Fiorentina in that game and also set up Khouma Babacar for another.
The Egyptian will play alongside Mario Gomez in the Fiorentina attack with Joaquin and Borja Valero playing in support.
Viola defender Micah Richards, like Salah, will be returning to familiar territory because he is on loan from Manchester City.